(2011). Dangshan Su) origin in Dangshan County, Anhui Province, China. (A) Diagram of the VIGS technique used for infecting the P. bretschneideri fruits. 24, 4624–4631. A Chinese White Pear (Pyrus bretschneideri) BZR Gene PbBZR1 Act as a Transcriptional Repressor of Lignin Biosynthetic Genes in Fruits Yunpeng Cao 1,3,4, Dandan Meng 4, Xiaoxu Li 5, Lihu Wang 6*, Yongping Cai 4* and Lan Jiang 2* doi: 10.1038/ng.740, Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011). (2002). doi: 10.1016/j.molp.2017.03.006, Maher, C., Stein, L., Ware, D. (2006). Then, using PF05687.10 as probe, the proteins database of the five Rosaceae genomes was searched by HMMER 3.0 software (Mistry et al., 2013). The TransDetect Double-Luciferase Reporter Assay Kit (TRANSGEN, China) was used to detect the luciferase activity. In general, Ka/Ks < 1 indicates functional constrain with the negative/purifying selection, Ka/Ks > 1 suggests accelerated evolution with the positive selection, and Ka/Ks = 1 indicates the genes are drifting neutrally (Yang, 2007). The heterologous expression of the A. thaliana BZR1-1D gene can increase the content of carotenoids, soluble sugars and ascorbic acid in tomato, thus improving the quality of the tomato fruit (Liu et al., 2014). In addition to the exon–intron structure analysis of BZR genes, other conserved domains or motifs analyses might also be important for the diversity of BZR protein sequences from F. vesca, P. mume, P. persica, P. communis, and P. bretschneideri. (2017). The previously published articles showed that PEST domains are contributed to controlling protein stability (Yin et al., 2005). BZR transcription factors play essential roles in plant growth and environmental stimuli, and they are also the positive regulators of Brassinosteroid (BR) signal transduction in diverse plants. Previous studies have shown that stone cell content is a key factor affecting P. bretschneideri fruit flavor (Jin et al., 2013; Cao et al., 2016c). Right panel: distribution of conserved motifs and exon-intron structure. It is hardy to zone (UK) 5. Soc. Chú thích Liên kết ngoài. Nucleic Acids Res. Two Putative BIN2 Substrates Are Nuclear Components of Brassinosteroid Signaling. The spectrum from blue to red indicates the relative expression level from 0 to 6. (2013). A! Plums are one of the most economically important Rosaceae fruit crops and comprise dozens of species distributed across the world. Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato. A recently evolved isoform of the transcription factor BES1 promotes brassinosteroid signaling and development in Arabidopsis thaliana. welcome to rigd welcome to rigd. To isolate and identify the active ingredients, the ethanol extract was separated into petroleum ether, ethyl acetate, n-butanol, and aqueous fractions. Nothospecies: Pyrus × bretschneideri. doi: 10.1093/nar/gku1221, Mistry, J., Finn, R. D., Eddy, S. R., Bateman, A., Punta, M. (2013). As shown in Figure S3, most of the genes exhibited similar splicing patterns, such 63.8% (30/47) BZR genes contained one or two introns. Recommended citation 'Pyrus bretschneideri' from the website Trees and Shrubs Online (treesandshrubsonline. Sci. Accessed: 07-Oct-06. 43, 109–116. Figure S4 | Figure S4 Intraspecies microsynteny of BZR gene families in P. bretschneideri and P. communis. A large scale duplication event was defined as a region where three or more conserved homologs were located within ten genes upstream and downstream between genomes. These trees were divided into four groups, designated as A, B, C and D. Figure 2 Interspecies microsynteny of BZR genes in five Rosaceae genomes, including Fragaria vesca, Prunus mume, Prunus persica, Pyrus communis and Pyrus bretschneideri. F. vesca, P. mume, P. persica, and P. communis), the different number of orthologous gene pairs were detected, indicating the different loss rates of duplicated genes were occurred during evolution. Plant Sci. The species is hermaphrodite (has both male and female organs) and is pollinated by Insects. cv. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Tree 5–15 m. Bark thick, dark, coarsely furrowed. (2016b). All materials used in this study were collected from pear trees (Pyrus bretschneideri Rehd. Under photoperiodic control, seedlings were grown in incubator for 40 days. doi: 10.1126/science.1153917, Tang, W., Yuan, M., Wang, R., Yang, Y., Wang, C., Osesprieto, J. This was received from Yashiroda in 1925, and although it has a slight lean to avoid a neighbour, is hale and hearty, with dense branching to the base. (2007). BZR transcription factors are associated with plant cell growth and photomorphogenesis by interacting with the Phytochrome-interacting factor (PIF) family genes and DELLA genes (Gallegobartolome et al., 2012; Li et al., 2012; Oh et al., 2012). The DNA dye 4,6-diamidino-2-phenylindole (DAPI) staining was carried out to indict the nucleus. doi: 10.1073/pnas.1119992109. Pyrus_Communis Detail . Front. YuC wrote the paper. 111, 671–678. doi: 10.1186/gb-2013-14-4-r36, Letunic, I., Doerks, T., Bork, P. (2012). Methods 25, 402–408. Based on this analysis, we found that the majority of Ka/Ks ratios across coding regions were far less than one, except from several distinct peaks (i.e. Genes 7, 89. doi: 10.3390/genes7100089, Cao, Y., Han, Y., Meng, D., Li, D., Jin, Q., Lin, Y., et al (2016c). Bot. In total, we identified 34 intraspecies BZR genes involved in the interspecies microsynteny (Figure 3 and Table S2). doi: 10.1073/pnas.0912333107, Yin, Y., Wang, Z. Y., Moragarcia, S., Li, J., Yoshida, S., Asami, T., et al. DAPI (dye 4,6-diamidino-2-phenylindole) staining was performed to indicate the nucleus. Biol. (1997). Signaling 5, ra72. WELCOME TO RIGD. Distinct transcriptome profiles reveal gene expression patterns during fruit development and maturation in five main cultivated species of pear (Pyrus L.). These genes were selected, because they had relatively higher expression than other genes in PbBZR1 mediated RNAi fruits. Science 320, 486–488. 162, 1965–1977. Cloning and characterization of BES1/BZR1 transcription factor genes in maize. Mol. To sum up, the structural analysis of BZR genes supported the above result from phylogenetic analysis. Pairwise protein sequence was first aligned using MUSCLE software, then the multiple sequence alignments of amino acid and the corresponding CDS sequences were converted to codon alignments using MEGA 5.0 software(Tamura et al., 2011). Front. Identity Taxonomic Tree List of Pests Uses List Summary. Science 307, 1634–1638. Group C contains the fewest BZR genes (five), while group A has the most BZR genes (seventeen), followed by group B (fourteen) and group D (eleven). 16, 227–233. Acad. The transcription repressing activities were indicated as the relative ratio of LUC/REN. Table 1 Duplicated BZR gene pairs in Pyrus bretschneideri and Pyrus communis. Fragaria_vesca Detail . Nat. (2011). MYB transcription factors in chinese pear (Pyrus bretschneideri Rehd. Figure S2 | Exon-intron structure and distribution of conserved motifs of BZR genes. Plant Sci. Loài này được Rehder miêu tả khoa học đầu tiên năm 1915. CDD: NCBI’s conserved domain database. (2010). Identification and characterization of the BZR transcription factor family and its expression in response to abiotic stresses in Zea mays L. Plant Growth Regul. , pyrus × bretschneideri ) trong họ hoa hồng pairs for all the constructs driven. Content of the five Rosaceae species loading these files, the Ks values of all paralogous pairs varied 0.1672... Fruit Lignification Toolbox in pear Reveals Targets for fruit Quality Bioengineering PbCCoAMOT1, PbCOMT3 and. Pp2C-1 Allele Underlying a quantitative Trait Locus enhances Soybean 100-Seed Weight are also two nice trees the. Then these target genes were cloned into the pTRV2 vector, respectively Cao Y.... The 4-Coumarate: Coenzyme a Ligase ( 4CL ) Related genes and expression of... 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